How to test nitrate-nitrogen (NO3-N) using the Kyoritsu packtest
What is it? Nitrates are a specific form of nitrogen that are formed after nitrites have been exposed to nitrifying bacteria. Nitrates (NO3) are bioavailable, making them an important source of nitrogen for plants.
Why is it important? Algae and other aquatic plants use nitrates as an important source of food. However, excess nitrates in water can make it difficult for aquatic insects and fish to survive. If we do not manage levels and understand the nitrogen cycle, algae growth can go unchecked, reducing dissolved oxygen in the water. This directly impacts aquatic life.
About the Kyoritsu test
How to test
- Take one tube out of the bag with the green label (Nitrates). Pull out the plastic pin. Squeeze out the air from the tube.
- Holding the squeezed tube, put it into your sample and release to suck up water (like a baster!). Your sample container should be half full.
- Invert at least 5 times to allow the reagent to dissolve in your sample. Set your timer for 3 minutes.
- Compare your results to the comparison chart. If the results are in between, record the halfway point.
Comparing results
Use the comparator provided with your kit and take your time. You're looking for if values are low/medium/high, rather than precision (not possible with this type of test)! Do not compare with a screenshot or photograph, as lighting will make it inaccurate.
Interpreting results
Normal levels depend on your location. The following values apply to the UK:
- 0.2 - 0.05 ppm: Low/Normal
- 0.05 - 0.1 ppm: Medium
- 2 - 5 ppm: High
- 5 - 10 ppm: Very high
Watch the protocol in action